Description

In-depth hormone testing to assess production, metabolism, and detoxification pathways

This advanced urine hormone test provides a detailed analysis of sex hormones and their metabolites — including estrogen, progesterone, testosterone, DHEA, and their breakdown products. It helps evaluate how hormones are produced, processed, and cleared from the body, offering critical insights into hormonal imbalances and long-term health risks.

At-home urine collection kit shipped nationwide

What’s Measured

This test includes over 40 biomarkers, organized into key hormone categories:

Estrogens & Estrogen Metabolites

These markers evaluate estrogen balance, detoxification, and cancer risk potential.

  • E1 (Estrone): A weaker estrogen, important after menopause.
  • E2 (Estradiol): The most active form of estrogen.
  • E3 (Estriol): A protective estrogen, especially during pregnancy.
  • 2-OH-E1 / 2-OH-E2: “Good” metabolites that support detoxification and are less estrogenic.
  • 4-OH-E1 / 4-OH-E2: Reactive metabolites that may damage DNA if not properly cleared.
  • 16α-OH-E1: Highly estrogenic; elevated levels can contribute to estrogen dominance.
  • 2-Methoxy-E1 / 2-Methoxy-E2: Detoxified forms of estrogens showing methylation activity.
  • 4-Methoxy-E1 / 4-Methoxy-E2: Methylated forms of more harmful 4-hydroxy estrogens.
  • 2-MeO-E1 / 2-OH-E1 Ratio: Assesses methylation efficiency.
  • 2-OH(E1 + E2) / 16α-OH-E1 Ratio: Evaluates balance between protective and proliferative pathways.

Progesterone & Neurosteroid Metabolites

These metabolites reflect the body’s progesterone levels and its calming neurosteroid effects.

  • 20α-Dihydroprogesterone: A pregnancy-supporting metabolite.
  • 3α-Dihydroprogesterone: A neuroactive progesterone derivative that promotes relaxation.
  • Allopregnanolone: Influences mood and sleep; known for anti-anxiety effects.
  • Allopregnanediol: A downstream neurosteroid metabolite.
  • Pregnanediol: A direct indicator of progesterone levels and metabolism.

Androgens & Metabolites

These markers assess testosterone, DHT, and androgen metabolism in both men and women.

  • Testosterone: Central to libido, muscle mass, mood, and energy.
  • Epi-Testosterone: Natural isomer used to assess balance.
  • T/Epi-T Ratio: Used to detect exogenous testosterone use or production imbalance.
  • 5α-DHT (Dihydrotestosterone): A potent androgen; involved in prostate health, skin, and hair.
  • 5α,3α-Androstanediol: A DHT metabolite; reflects androgenic activity.
  • Androstenedione: A precursor to both estrogen and testosterone.
  • Androsterone: A 5α-reduced androgen metabolite.
  • Etiocholanolone: A 5β-reduced metabolite, less androgenic and more anti-inflammatory.
  • Androsterone / Etiocholanolone Ratio: Indicates activity of the 5α-reductase enzyme.

Adrenal & Stress-Related Steroids

These biomarkers provide insight into adrenal function, stress response, and glucocorticoid metabolism.

  • Cortisol: The primary stress hormone, affects energy, immunity, and inflammation.
  • Cortisone: Inactive form of cortisol; provides insight into stress hormone regulation.
  • Cortisol / Cortisone Ratio: Measures conversion between active and inactive cortisol.
  • Total Cortisol / Total Cortisone: Sum total output of glucocorticoids.
  • Corticosterone: A precursor to aldosterone, involved in blood pressure regulation.
  • Deoxycorticosterone: Supports electrolyte balance; linked to adrenal gland activity.
  • DHEA: An adrenal hormone and precursor to sex hormones. Vital for energy and mood.

Additional Biomarkers

Other compounds that affect hormone activity and test reliability.

  • BPA (Bisphenol A): A known endocrine disruptor; may interfere with hormone signaling.
  • Creatinine: Ensures urine concentration is within a valid range.
  • E3 / (E1 + E2) Ratio: Evaluates the proportion of protective estrogens in the total estrogen pool.

Who Should Consider This Test?

You may benefit from this panel if you are experiencing:

  • PMS, PCOS, perimenopause, or menopause
  • Low libido, irregular cycles, or mood changes
  • Fatigue, sleep disturbances, or hormone therapy monitoring
  • Symptoms of estrogen dominance or androgen excess
  • Concerns about hormone detoxification and long-term risk
  • Suspected effects of BPA or environmental hormone disruptors

Test Overview

  • Test Type: Urine
  • Collection Method: At-home kit (includes detailed instructions)
  • Shipping: Nationwide (USA)